Coding

Part:BBa_K5271003:Design

Designed by: Yin Yan Chan   Group: iGEM24_HKPOLYU   (2024-09-28)


HER2-binding peptide -scrambled


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our preliminary results when it is joined with the EGFR nanobody by a linker, the linker should avoid cysteine residues since it potentially reduces the solubility of the dual targeting nanobody in prokaryotic expression system.

Source

The sequence of the HER2-binding peptide -scrambled was randomly generated and had a same length with the HER2 binding region of Panobody.

References

  • Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., ... & Hassabis, D. (2021). Highly accurate protein structure prediction with AlphaFold. nature, 596(7873), 583-589.